Polygain reduces methane-producing microbes in spelling horses

Summary

  • The relative abundance of methane-producing bacteria was over 60% lower after Polygain was added to the diet. The methane-producing bacteria abundance returned to prior levels after Polygain supplementation stopped.

  • Species of gut bacteria that increased included those associated with increased production of short-chain fatty acids

Trial Design

The diversity of the equine hindgut microbiome was explored through the analysis of PCR-amplified 16S ribosomal V3-V4 gene sequences. 120 faecal samples were collected from eight horses over a twelve-week period prior to, during and following the removal of Polygain. Each horse was paddocked alone and had free access to pasture. They were fed additional Lucerne and grass hay in the morning and evening.

Results

A clear reduction in methane-producing bacteria during the month where Polygain was added to the diet.

The bacteria associated with the production of valuable short-chain fatty acids, such as Alistipes and Enterobacter, were found to increase.

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